Anti-Rock2 Antibody

Rho-associated coiled-coil kinase 2 (ROCK2) is a serine/threonine kinase that operates downstream of RhoA GTPase, governing actin cytoskeleton remodeling, actomyosin contractility, focal adhesion turnover, and stress fiber formation. This unconjugated rabbit polyclonal reagent from Antibodies.com is raised against ROCK2 and supplied as a 50 µl vial at 1 mg/ml in phosphate buffered saline containing 15 mM sodium azide at approximately pH 7.2, validated for Western blot detection of endogenous kinase in human, mouse, and rat sample lysates.

Researchers investigating vascular smooth muscle contraction, cardiac hypertrophy, neuronal axon guidance, glaucoma-associated fibrosis, and tumor cell invasion rely on this polyclonal antibody to monitor kinase expression and the phosphorylation status of downstream substrates including myosin light chain phosphatase, cofilin, and LIM kinase. Aliquot upon first use and store refrigerated; repeated freeze-thaw cycles reduce binding activity. The antibody recognizes the endogenous ROCK2 band at approximately 160 kDa in whole-cell extracts prepared with standard RIPA lysis buffer, supporting studies of RhoA/ROCK signaling in cardiovascular, neurological, and oncology research contexts.