Anti-ROCK1 Antibody

Rho-associated coiled-coil kinase 1 (ROCK1) is a ~158 kDa serine/threonine kinase that acts as a major downstream effector of RhoA GTPase signaling, regulating actin cytoskeleton dynamics, stress fiber assembly, focal adhesion turnover, and smooth muscle contraction. The Antibodies.com Anti-ROCK1 polyclonal antibody is a rabbit host Primary Antibody supplied as 100 µl of unconjugated reagent, validated for Western blot, immunohistochemistry, and immunofluorescence detection of human ROCK1.

This reagent targets a protein of intense research interest because ROCK1 phosphorylates substrates including MYPT1, myosin light chain (MLC), and LIM kinase, coupling RhoA activity to actomyosin contractility, cell migration, and cell shape changes. Altered ROCK1 signaling has been implicated in vascular tone regulation, cardiac remodeling, glaucoma pathophysiology, and tumor cell invasion, making this antibody useful across cardiovascular research, cancer biology, neuroscience, and ophthalmology studies. The unconjugated format offers flexibility in choosing secondary antibodies tailored to specific detection chemistries across WB, IHC, and IF workflows.

Provided in a vial suitable for aliquoting, the antibody should be stored refrigerated to preserve activity, with aliquots recommended to limit freeze-thaw cycles. Its polyclonal design enables recognition of multiple epitopes on human ROCK1, which can be advantageous for detecting full-length protein or the 130 kDa caspase-3-cleaved fragment that appears during apoptosis, expanding the antibody's utility in cell death and signaling studies.