Anti-Rock-2 Antibody

This polyclonal antibody from Antibodies.com is raised in rabbit against Rho-associated coiled-coil kinase 2 (ROCK2), a serine/threonine kinase that acts downstream of RhoA GTPase to phosphorylate substrates involved in actin cytoskeleton organization, stress fiber formation, focal adhesion turnover, and cell migration.

The reagent is supplied as 100 µl of a 1 mg/ml stock in PBS containing 0.1% sodium azide and 50% glycerol, giving ample volume for routine western blotting, immunohistochemistry, immunofluorescence, and immunoprecipitation protocols. Validated reactivity covers human, mouse, and rat samples, supporting studies of vascular smooth muscle contraction, neurite retraction, cytokinesis, and ROCK-pathway involvement in cancer metastasis, fibrosis, and cardiovascular disease models.

Researchers probing phosphorylation events at consensus ROCK substrates such as MYPT1, MLC2, and LIMK rely on unconjugated primaries like this for flexible secondary antibody selection and signal amplification. Sodium azide in the buffer prevents microbial contamination, while the 50% glycerol cryoprotectant allows storage at -20 °C without freeze-thaw damage across many freeze-thaw cycles.