Anti-ROBO3 Antibody

ROBO3 (Roundabout homolog 3) is a transmembrane axon guidance receptor that helps commissural neurons cross the midline of the developing brainstem and spinal cord through the Slit-Robo signaling pathway. Biallelic loss-of-function mutations in the human gene are linked to horizontal gaze palsy with progressive scoliosis (HGPPS), and the protein is studied in CNS developmental neurobiology, floor-plate signaling, and axon pathfinding research.

This 200µl liquid aliquot from Antibodies.com is a rabbit polyclonal IgG reagent supplied unconjugated for western blot detection of endogenous ROBO3 in mouse and rat samples. The polyclonal mix recognizes multiple epitopes along the ROBO3 cytoplasmic tail, an advantage when probing post-translational modifications or distinguishing isoforms.

For western blot, the unconjugated format lets researchers pair the primary with their own HRP, fluorophore, or biotin secondary reagents. The reagent is validated for mouse and rat reactivity and is used with embryonic and postnatal rodent CNS tissue. Store refrigerated and avoid repeated freeze-thaw cycles; aliquot upon first thaw if working volume will not be used quickly. For research use only.