Anti-ROBO3 Antibody

From Antibodies.com, this polyclonal reagent is a rabbit IgG raised against ROBO3 (Roundabout Guidance Receptor 3), a single-pass transmembrane receptor of the immunoglobulin superfamily that mediates SLIT-dependent repulsion of commissural axons at the midline of the developing central nervous system. The antibody is supplied unconjugated in a 100 µL volume and has been validated for Western blot detection of endogenous ROBO3 protein in mouse and rat tissue lysates. Because ROBO3 is expressed as two principal splice isoforms, ROBO3.1 and ROBO3.2, that are deployed on opposite sides of the midline to govern axon entry and prevent recrossing, the polyclonal format offers broad epitope coverage across developmental stages where isoform balance shifts between spinal cord and hindbrain regions. Loss-of-function mutations in the ROBO3 gene cause horizontal gaze palsy with progressive scoliosis (HGPPS), an autosomal recessive disorder in which corticospinal and somatosensory tracts fail to decussate at the medulla, making this reagent relevant to neurodevelopmental disease research. The antibody is also used in oncology studies where deregulated SLIT/ROBO signaling has been implicated in tumor cell migration, invasion, and angiogenesis. For long-term activity, store the working aliquot at -20 °C and avoid repeated freeze-thaw cycles to preserve IgG binding capacity.