Anti-RNF8 Antibody

This Rabbit polyclonal primary antibody from Antibodies.com is raised against RNF8 (Ring Finger Protein 8), an E3 ubiquitin ligase that plays a central role in the cellular DNA damage response. RNF8 catalyzes the ubiquitination of histone H2A at DNA double-strand break sites, a modification that is required for the downstream recruitment of repair factors including BRCA1 and 53BP1 to damaged chromatin, working in concert with UBC13 to amplify the ubiquitin signal at lesion sites. The unconjugated reagent is supplied in a 100 µl volume and is validated for Western blot (WB) and immunohistochemistry (IHC) workflows on human samples. Investigators studying genome stability, ATM-dependent signaling, ubiquitin-mediated chromatin remodeling, or the molecular basis of homologous recombination repair will find this antibody useful for examining endogenous RNF8 expression levels and modification patterns in whole-cell lysates, nuclear extracts, and formalin-fixed tissue sections. Its polyclonal format provides multiple epitope recognition, supporting robust signal detection across a range of experimental conditions relevant to DNA repair and checkpoint research.