Anti-RNF7 Antibody

RNF7 (also known as ROC2 or SAG) is a RING finger domain protein that forms the catalytic core of Cullin-RING E3 ubiquitin ligase complexes, where it drives ubiquitin transfer onto substrate proteins destined for proteasomal turnover. From Antibodies.com, this rabbit polyclonal reagent recognizes endogenous RNF7 in both human and mouse samples and is validated for western blot and immunohistochemistry workflows.

The antibody is supplied as 100 µl of unconjugated antiserum, ready to pair with HRP- or fluorophore-labeled secondary reagents for chemiluminescent or fluorescent detection. On western blots, RNF7 is typically resolved as a band near 12-13 kDa, consistent with the calculated mass of the unmodified protein, while immunohistochemistry staining localizes the protein predominantly to the cytoplasm and, in some cell types, to the nucleus. For best results, aliquot the reagent upon first use to minimize freeze-thaw cycles. Laboratories studying the VHL/HIF axis, hypoxia signaling, apoptosis regulation, or ubiquitin-mediated protein turnover use this reagent to monitor Cullin-RING ligase activity in cell lysates and tissue sections.