Anti-RNF7 Antibody

RNF7 (also called SAG or ROC2) is a small RING-domain E3 ubiquitin ligase that operates as a core component of Cullin-RING ligase complexes, driving ubiquitin-mediated protein degradation. This polyclonal antibody from Antibodies.com is raised in rabbit and validated for sensitive detection of endogenous RNF7 across multiple experimental platforms. Supplied as an unconjugated reagent, it offers flexibility to pair with a researcher's preferred secondary detection system.

The product is provided as a 100 µl aliquot at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, a formulation that supports refrigerated storage and repeated freeze-thaw handling without appreciable loss of activity. The polyclonal nature of the reagent broadens epitope coverage, which can be advantageous when probing conformational or post-translationally modified forms of the 12.7 kDa RNF7 protein.

Validated applications include Western blot, immunohistochemistry, and immunofluorescence, with confirmed reactivity against human, mouse, and rat samples. The antibody is therefore suitable for cross-species comparative studies of E3 ligase expression, subcellular localization in cultured cells, and tissue-level distribution analysis. Investigators studying apoptosis regulation, cell cycle control, oxidative stress signalling, and proteasome biology frequently use anti-RNF7 reagents to monitor pathway activity and substrate turnover.