Anti-RNF133 Antibody

This rabbit polyclonal IgG reagent from Antibodies.com is raised against RNF133, a testis-specific RING-type E3 ubiquitin-protein ligase (UniProt Q8WVZ7) that functions in ER-associated protein ubiquitination and is required for male fertility. The unconjugated antibody is supplied as a 100 µl liquid aliquot in a single vial, validated for western blot detection of the human target in cell lysates prepared under standard reducing SDS-PAGE conditions before transfer to nitrocellulose or PVDF membranes. RNF133 contains a C3H2C3 RING finger domain together with a protease-associated domain, signal peptide, and transmembrane region, and physically partners with the E2 conjugating enzyme UBE2J1 during spermatid maturation. Working stocks should be refrigerated at 4 °C for short-term use; for longer storage, freeze at -20 °C in single-use aliquots to limit freeze-thaw cycles and preserve binding activity. The reagent is intended for research use only and is shipped without preservative, ready for downstream HRP- or fluorophore-conjugated secondary antibody workflows in basic research laboratories studying reproductive biology and ubiquitin pathway signaling.