Anti-RIP3 Antibody

RIPK3 is a serine/threonine kinase central to necroptotic signaling, where it partners with RIPK1 to drive formation of the necrosome and subsequent MLKL-mediated membrane rupture. This rabbit polyclonal reagent from Antibodies.com targets the RIP3 kinase domain and is validated for western blot and immunohistochemistry on mouse-derived samples, including paraffin-embedded tissue sections and whole-cell lysates. The antibody is supplied as a 100 µl aliquot at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a cryoprotectant, and should be stored at -20 °C in working aliquots to limit freeze-thaw cycles. Researchers studying TNF-driven inflammation, ischemic injury, and viral-infection models use the reagent to monitor endogenous RIPK3 expression and to discriminate necroptosis from caspase-dependent apoptosis in cell culture and in vivo systems. As an unconjugated polyclonal, the antibody is also compatible with custom secondary-labeling strategies for immunofluorescence, immunoprecipitation, and pull-down workflows.