Anti-RING1 Antibody

Raised in rabbit and affinity-purified as an unconjugated IgG polyclonal reagent, this 100 µl antibody from Antibodies.com recognizes the RING1 protein (also known as RING1A or RNF1), a 42 kDa E3 ubiquitin-protein ligase and core subunit of the Polycomb Repressive Complex 1 (PRC1). The reagent has been validated for Western blot detection in human and rat samples, where it produces a clear band at the expected molecular weight of approximately 42 kDa. RING1 catalyzes monoubiquitination of histone H2A at lysine 119 (H2AK119ub), an epigenetic mark associated with transcriptional repression, X-chromosome inactivation, and the maintenance of stem cell pluripotency. The polyclonal format provides epitope diversity, supporting robust signal even when individual linear epitopes are altered by denaturation or post-translational modification during sample preparation. The vial is shipped frozen and should be stored at -20 degrees C to preserve activity, with aliquoting recommended to limit freeze-thaw cycles. Researchers investigating Polycomb-mediated gene silencing, chromatin remodeling, or developmental epigenetic signaling can apply this reagent to whole-cell lysates and tissue extracts. The product is intended for research use only and is not validated for diagnostic or therapeutic applications.