Anti-Ribosomal Protein L27A Antibody

Produced in rabbit as an unconjugated polyclonal reagent, this primary antibody from Antibodies.com recognizes Ribosomal Protein L27A (RPL27A), a component of the large 60S ribosomal subunit involved in ribosome biogenesis and cytoplasmic protein translation. Validated for Western blot, immunohistochemistry, immunofluorescence, and ELISA workflows, the same lot can be used for both biochemical quantitation in cell lysates and spatial localization in tissue sections.

Reactivity is confirmed for Human, Mouse, and Rat samples, which supports translational studies that move between patient-derived material and rodent model tissues. Each vial ships with 100 µL of antiserum, typically enough for multiple Western blot membranes and a series of IHC staining runs once working dilutions are optimized. RPL27A is constitutively expressed in proliferating cells and is frequently used as a loading reference or to monitor shifts in ribosomal protein stoichiometry under differentiation, cellular stress, or pharmacological treatment conditions.

For handling, the antibody is generally stored refrigerated for short-term use and aliquoted before freezing to limit repeated freeze-thaw cycles. Intended for research use only.