Anti-RHOT1 Antibody

Antibodies.com provides a rabbit polyclonal antibody that recognises RHOT1, a protein also referred to as MIRO1. The reagent is supplied as an unconjugated IgG in liquid form, raised against a recombinant fusion protein corresponding to amino acids 310–590 of human MIRO1, and purified by affinity chromatography. It reacts with Human, Mouse, and Rat samples and has been validated for Western blot, with the catalogue also listing IHC and the manufacturer additionally listing ELISA as a validated application. Recommended starting dilutions are 1:2,000–1:6,000 for WB and 1 µg/ml for ELISA. The product is reported at 80 kDa, with a predicted molecular weight of 71 kDa. It is formulated in phosphate buffered saline (pH 7.3) containing 50% glycerol and 0.02% sodium azide, and is KD validated with supporting scientific images. The concentration is lot-specific. Storage instructions call for shipping at 4°C, aliquoting on arrival, and storage at -20°C, avoiding repeated freeze/thaw cycles.