Anti-RHOB Antibody

Rabbit polyclonal antibody targeting RHOB (also referred to as RhoB), offered by Antibodies.com as a primary antibody reagent. Raised in rabbit, the antibody is of the IgG isotype and is supplied unconjugated. Western blot (WB) is listed as a validated application, with ELISA additionally noted by one manufacturer source and immunohistochemistry (IHC) noted in the Antibodies.com catalogue listing. Reported reactivity covers Human and Mouse samples, with manufacturer sources additionally listing Rat.

The immunogen is a synthetic peptide corresponding to a region within amino acids 70–150 of human RhoB (NP_004031.1), and the target protein has a reported molecular weight of 21 kDa. The antibody is affinity-purified and supplied as a liquid. One source describes formulation in Phosphate Buffered Saline, pH 7.3, containing 50% glycerol and 0.05% Proclin 300; another describes PBS pH 7.4 with 150 mM NaCl, 0.02% sodium azide and 50% glycerol. Concentration is reported as lot specific in one source and 1.0 mg/mL in another. Recommended Western blot dilution is 1:500–1:1,000. The product is shipped at 4°C and should be aliquoted upon delivery and stored at –20°C, avoiding freeze/thaw cycles.