Anti-Rho A Antibody

Manufactured by Antibodies.com, this rabbit polyclonal reagent is raised against the Rho A GTPase and is validated for Western blotting, immunohistochemistry and ELISA applications. Supplied as a 50 µl liquid aliquot at 1 mg/ml in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, the antibody is unconjugated and of the IgG isotype, allowing flexible downstream labelling. The immunogen is a synthetic peptide derived from human Rho A centred on the non-phosphorylation site at S188, and the antiserum has been affinity-purified by epitope-specific affinity chromatography, ensuring selective recognition of endogenous Rho A levels in human, mouse and rat samples. Because the reagent is polyclonal, it offers broad epitope coverage, which is particularly useful when studying Rho A signalling, cytoskeletal regulation and small GTPase biology across multiple model systems. For optimal stability, the vial should be stored at –20 °C, where it remains viable for up to one year, and repeated freeze-thaw cycles should be avoided to preserve binding activity. Researchers working on cell migration, invasion, adhesion or GTPase-driven oncogenic pathways can rely on this lot for reproducible detection in lysates and tissue sections, and the unconjugated format also makes it compatible with secondary antibody detection chemistries of their choice.