Anti-RFC1 Antibody

An affinity-purified rabbit polyclonal reagent raised against RFC1 (Replication Factor C Subunit 1), the largest subunit of the heteropentameric clamp loader that assembles PCNA onto primed DNA during S-phase replication and supports DNA repair pathways such as nucleotide excision repair and mismatch repair. The unconjugated format allows flexible pairing with HRP, biotin, or fluorophore-conjugated secondary antibodies depending on the chosen detection platform.

Validated by the supplier for Western blot, immunohistochemistry on formalin-fixed paraffin-embedded sections, and immunofluorescence imaging. Reactivity is reported across human, mouse, and rat samples, supporting translational work between rodent disease models and clinical specimens. Each vial from Antibodies.com is supplied as 100 µL of liquid antibody, sufficient for multiple membrane probing and staining experiments when used at standard working dilutions.

RFC1 serves as a useful marker in studies of replication stress, genotoxic stress responses, and cell-cycle checkpoint control, and has gained relevance in neurogenetics research following the identification of intronic RFC1 repeat expansions as a major genetic cause of cerebellar ataxia with neuropathy and vestibular areflexia syndrome (CANVAS). The polyclonal host response against multiple epitopes can be advantageous when probing denatured targets on membranes or fixed tissue, where recognition of linearized determinants improves signal consistency.