Anti-RFC1 Antibody

RFC1 (Replication Factor C subunit 1) is the largest subunit of the heteropentameric RFC complex that loads PCNA onto primed DNA during replication and repair. This unconjugated rabbit polyclonal antibody from Antibodies.com recognises endogenous RFC1 in human, mouse, and rat samples and has been validated for Western blot, immunohistochemistry, and immunofluorescence workflows.

Each vial contains 100 µl of antibody at 1 mg/ml, formulated in PBS with 0.1% sodium azide and 50% glycerol. The polyclonal format broadens epitope coverage across RFC1 isoforms and post-translational states, which is useful when probing chromatin-associated pools of the clamp loader during S-phase, replication stress, or genotoxic challenge.

RFC1 supports lagging-strand synthesis, nucleotide excision repair, and the DNA damage checkpoint, so researchers in DNA replication, genomic stability, and cancer biology fields use this reagent to track loading-complex behaviour and PCNA-dependent repair activity in cell and tissue models.