Anti-RELA (phospho T435) Antibody

RELA (p65) is the canonical transactivating subunit of the NF-κB transcription factor complex, and phosphorylation at threonine 435 within its C-terminal transactivation domain modulates co-activator recruitment and downstream gene expression. This unconjugated rabbit polyclonal IgG reagent specifically detects the phospho-T435 form of RELA in human samples and has been validated for Western blot, immunohistochemistry, and immunofluorescence workflows.

Supplied as 200 µl of ready-to-use reagent, the antibody supports visualization of activated NF-κB signaling in fixed cells, tissue sections, and lysates. Researchers studying inflammation, innate immunity, oncogenic signaling, or cytokine-driven transcriptional programs use phospho-T435-specific detection to track pathway engagement independently of total RELA levels, providing insight into stimulus-dependent regulation of the transcription factor. Storage at -20 °C in small aliquots is recommended to preserve phospho-epitope binding across multiple freeze-thaw cycles, with working stocks refrigerated for short-term use.

Manufactured by Antibodies.com, this primary antibody reagent has a Rabbit host, IgG isotype, polyclonal clonality, unconjugated format, and reactivity against human RELA. It is intended for research use only and supports mechanistic studies linking upstream kinase activity to NF-κB-dependent gene regulation.