Anti-RELA (phospho S536) Antibody

Produced by Antibodies.com, this rabbit polyclonal antibody recognises the phosphorylated form of NF-kB p65 (RELA) at serine 536. The unconjugated IgG reagent is supplied as a liquid preparation and is reported to react with human, mouse and rat samples.

According to the manufacturer's data, the antibody has been validated for Western blotting and is recommended for use at working dilutions between 1:2,000 and 1:10,000. Catalogue information also lists immunohistochemistry and immunofluorescence as supported applications. The immunogen corresponds to a synthetic phosphorylated peptide spanning the S536 region of human RelA (NCBI accession NP_068810.3), with the core sequence FSSIA.

The target protein has an expected molecular weight of 65 kDa. The antibody is affinity-purified and formulated in phosphate buffered saline (pH 7.3) containing 50% glycerol and 0.05% Proclin 300. It is shipped at 4 °C and should be aliquoted and stored at −20 °C, with freeze/thaw cycles to be avoided. The reagent is supplied at a lot-specific concentration and is accompanied by three scientific images.