Anti-RELA (phospho S529) Antibody

Phosphorylation of the RELA subunit of NF-κB at serine 529 is implicated in transactivation activity downstream of IKK and casein kinase II signaling pathways. This rabbit polyclonal IgG reagent from Antibodies.com is raised against the phospho-S529 epitope and supplied as an unconjugated preparation at 50 µl per vial, calibrated for western blot detection of the modified human p65 protein. In western blot, the antibody recognizes endogenous RELA in lysates from human cell lines and primary cultures, producing a discrete band at approximately 65 kDa consistent with the full-length transcription factor. Researchers commonly apply it to signaling time-course studies following TNF-α, IL-1β, or LPS stimulation, where it discriminates the activated phospho-pool from total p65. The reagent should be stored frozen in small aliquots to limit freeze-thaw cycles and protected from light to preserve binding activity. Pair this phospho-specific reagent with a pan-RELA or β-actin loading control to normalize phosphorylation readouts across experimental conditions.