Anti-REC8 Antibody

REC8 (meiotic recombination protein 8) is a meiosis-specific component of the cohesin complex that maintains sister-chromatid cohesion from DNA replication through anaphase II, and is essential for synaptonemal complex assembly, crossover resolution, and ordered homolog segregation during gametogenesis. The Anti-REC8 Antibody from Antibodies.com is a rabbit polyclonal reagent raised against human REC8 epitopes, supplied as 100 µl of unconjugated, preservative-stabilized liquid suitable for direct labeling or secondary-detection workflows.

Validated applications include Western blot (WB) and immunohistochemistry (IHC) on human samples. Common uses are immunoblotting of lysates from meiotic or induced-meiotic cell systems to confirm REC8 expression and post-translational modification status, and IHC on fixed testis or ovary sections to localize REC8 within spermatocyte and oocyte nuclei across meiotic prophase I substages. The reagent is also frequently paired with STAG3 or SMC1β antibodies to map cohesin sub-complex assembly in germ cells.

For handling, aliquot upon receipt to limit freeze-thaw cycles, store refrigerated in the dark, and protect from light during use. Brief equilibration to room temperature before pipetting reduces viscosity and improves reproducibility. Short-term stability is retained under cold-chain conditions; for long-term storage, keep frozen in single-use aliquots.