Anti-REC8 Antibody

Raised in rabbit against the meiotic recombination protein REC8, this polyclonal IgG reagent from Antibodies.com detects a core component of the cohesin complex that holds sister chromatids together during meiosis. REC8 loading and removal are tightly linked to homologous recombination, crossover resolution, and accurate chromosome segregation in germ cells, so this antibody is widely used in reproductive biology and meiosis research.

The unconjugated antibody is supplied in a 100 µl volume and is validated for Western blot (WB) applications on samples of human and rat origin. Because the polyclonal host response recognizes multiple epitopes on REC8, the reagent can amplify signal from low-abundance meiotic proteins in testicular or ovarian lysates separated by SDS-PAGE and transferred to nitrocellulose or PVDF membranes.

Common downstream readouts include chemiluminescent or HRP-based detection of REC8 across meiotic prophase I substages, supporting studies of spermatogenesis, oogenesis, double-strand break processing, and the DNA damage response in germ cell development. Researchers also pair it with stage-specific markers to track REC8 dynamics from leptotene through the diplotene-to-diakinesis transition.