Anti-RBX1 Antibody

This polyclonal antibody from Antibodies.com targets RBX1, a core RING finger component of Cullin-RING E3 ubiquitin ligase complexes. Raised in rabbit and supplied unconjugated in a 100 µl vial, it is validated for Western blot and immunofluorescence workflows across human, mouse, and rat samples, supporting studies of ubiquitin-dependent proteolysis, cell cycle transitions, and cullin neddylation dynamics.

RBX1 (also called ROC1) scaffolds the catalytic core of SCF and related CRL complexes by binding E2 ubiquitin-conjugating enzymes and positioning them for substrate transfer. Because of its central role in targeting cell cycle regulators, transcription factors, and DNA damage response proteins for proteasomal degradation, the antibody is widely used in research on checkpoint control, signal transduction, and cancer biology. Researchers also apply it to investigate neddylation, deneddylation by the COP9 signalosome, and small molecule inhibition of Cullin-RING ligases.

As a primary antibody reagent for research use only, the 100 µl volume provides ample material for multiple blotting membranes or coverslip-based imaging experiments. Users commonly pair it with anti-rabbit secondary antibodies conjugated to HRP for chemiluminescent Western blot detection or to fluorophores for immunofluorescence imaging of fixed cells and tissue sections.