Anti-RBX1 Antibody

This unconjugated rabbit polyclonal antibody from Antibodies.com targets RBX1, a RING finger protein that functions as the catalytic core of Cullin-RING E3 ubiquitin ligase complexes. Supplied as a 100 µl vial of polyclonal antiserum, the reagent is validated for western blot and immunohistochemistry workflows and shows reactivity with human and mouse samples.

RBX1 (also called ROC1) partners with cullins and substrate adaptors to drive ubiquitin transfer onto cell-cycle regulators, transcription factors, and signaling proteins destined for proteasomal degradation. Researchers use the antibody to monitor SCF complex assembly, probe CRL-mediated turnover of substrates such as p27 and IκBα, and assess ligase activity in tumor biology and DNA damage response studies.

As an unconjugated primary reagent, the antibody pairs with HRP, biotin, or fluorophore-conjugated secondaries of the researcher's choice, supporting flexible detection across membrane-based and tissue-staining protocols. Aliquot upon receipt and refrigerate to preserve polyclonal stability across repeated use.