Anti-RBPJ Antibody

The RBPJ transcription factor, also called CBF1 or RBP-Jκ, is the central DNA-binding mediator of Notch signaling, where it toggles between repressor and activator complexes depending on NICD availability. This polyclonal rabbit antibody from Antibodies.com targets the RBPJ protein for detection across Western blot and immunohistochemistry workflows.

Raised in rabbit, the reagent is supplied as an unconjugated 100 µl aliquot and recognizes RBPJ in human, mouse, and rat samples, supporting cross-species studies of Notch-dependent transcriptional programs. The polyclonal format increases the likelihood of recognizing multiple epitopes on the protein, while the unconjugated presentation allows researchers to couple it to their preferred fluorophore or enzyme for custom detection schemes.

In fixed tissue sections, the antibody enables visualization of RBPJ distribution relevant to developmental biology, neurogenesis, and hematopoietic lineage commitment. In Western blot it permits evaluation of expression changes linked to Notch pathway activity, T-cell versus B-cell fate decisions, and the repressor-to-activator conversion of RBPJ-containing complexes. Investigators working on Notch signaling components, CBF1 co-repressor assemblies, or differentiation gene regulation can integrate this reagent into primary antibody panels for cell fate, immunology, and signaling research.