Anti-RBPJ Antibody

This rabbit polyclonal antibody targets RBPJ (recombination signal binding protein for immunoglobulin kappa J region), a key transcription factor in the Notch signaling cascade. Supplied by Antibodies.com as a 100 µl vial at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, the reagent supports western blot and immunohistochemistry workflows on human, mouse, and rat samples.

RBPJ, also referred to as CSL or CBF1, acts as the central DNA-binding mediator of canonical Notch signaling and is widely studied in lymphocyte development, vascular patterning, and oncogenic transcription programs. The unconjugated format allows researchers to pair the antibody with their preferred secondary detection reagent, including HRP, biotin, or fluorophore-conjugated anti-rabbit IgG.

The polyclonal clonality provides broad epitope recognition across multiple RBPJ isoforms, useful when probing cleavage variants produced by Notch receptor activation. For handling, store the vial refrigerated for short-term use, or aliquot and freeze at -20 °C for long-term stability, avoiding repeated freeze-thaw cycles to preserve binding activity.