Anti-RBL1 Antibody

Raised in rabbit as a polyclonal reagent, this unconjugated primary antibody from Antibodies.com is intended for western blot detection of RBL1 (retinoblastoma-like 1, also known as p107), a pocket-family tumor suppressor that governs the G1-to-S checkpoint alongside RB1 and RBL2. The IgG preparation is supplied at 1 mg/ml in phosphate buffered saline containing 15 mM sodium azide at approximately pH 7.2, and the 100 µl pack size supports multiple membrane exposures and titration experiments. Reactivity data confirm binding to the human, mouse, and rat orthologues of RBL1, enabling comparative work in established cell lines and primary tissue lysates across the three species. Researchers studying E2F-dependent transcription, CDK4/6 signaling, and the disruption of pocket-protein function in cancer models routinely probe RBL1 levels with this reagent; it is also useful for confirming CRISPR edits that truncate or knock out the RBL1 locus and for monitoring differentiation-associated shifts in p107 abundance. Because the antibody is unconjugated, it pairs with HRP-, AP-, or fluorophore-tagged secondary reagents of the user's choice, supporting chemiluminescent and multiplex fluorescence detection on standard PVDF or nitrocellulose membranes.