Anti-RBBP5 Antibody

This polyclonal antibody from Antibodies.com targets RBBP5 (Retinoblastoma-Binding Protein 5), a core scaffolding subunit of the MLL/SET1 (COMPASS) histone H3 Lys4 methyltransferase complex. Raised in rabbit and affinity-purified as an IgG preparation, the reagent is supplied unconjugated at 50 µl per vial and is validated for Western blot, immunohistochemistry, and immunofluorescence workflows across human, mouse, and rat samples.

RBBP5 functions as a structural platform that organizes the MLL/SET1 complex on chromatin, supporting H3K4 methylation and downstream transcriptional activation at developmental gene loci. The polyclonal reagent cross-reacts with the RBBP5 orthologues of human, mouse, and rat, enabling comparative studies across these species. Researchers apply it to probe complex assembly by co-immunoprecipitation, to map RBBP5 distribution in fixed tissue sections by IHC, and to localize the protein at subcellular sites by IF. Affinity purification provides lot-to-lot consistency, and the unconjugated format allows flexible pairing with secondary antibodies, HRP or fluorophore conjugates, or custom labeling strategies.

Investigators use this reagent in studies of embryonic development, hematopoiesis, and cancer biology, where altered H3K4 methylation patterns and MLL complex activity are of mechanistic interest. The 50 µl volume supports multiple experimental replicates, and the polyclonal antibody mixture recognizes multiple epitopes on RBBP5 for consistent signal in whole-cell lysates, paraffin-embedded tissue sections, and cultured mammalian cells.