Anti-RBBP5 Antibody

Raised in rabbit against retinoblastoma-binding protein 5 (RBBP5), this unconjugated polyclonal IgG reagent from Antibodies.com is supplied as a 100 µl aliquot validated for western blot, immunohistochemistry, and immunofluorescence workflows. RBBP5 acts as a core scaffolding subunit of the MLL/SET1 (COMPASS) histone H3 lysine 4 methyltransferase complex, where it stabilizes the catalytic SET1/MLL proteins and supports global H3K4 trimethylation across active promoters. Cross-reactivity is confirmed for mouse and rat orthologs alongside the human target, enabling comparative chromatin studies in standard laboratory models. Typical uses include profiling COMPASS complex assembly on chromatin, tracking H3K4 methylation changes during stem-cell and hematopoietic differentiation, and characterizing leukemia cell lines harboring MLL rearrangements. Aliquot upon receipt and refrigerate to limit repeated freeze-thaw cycles; pair with appropriate HRP- or fluorophore-conjugated secondary reagents for detection.