Anti-RB1 Antibody

This rabbit polyclonal antibody from Antibodies.com targets the retinoblastoma protein (RB1), a well-characterized tumor suppressor that governs the G1/S checkpoint of the cell cycle by binding E2F transcription factors. Raised in rabbit and purified as an IgG polyclonal, the reagent is supplied unconjugated in a 200 µl vial, allowing researchers to pair it with their own secondary antibodies and detection chemistries for Western blot detection of endogenous mouse RB1. The reagent is well suited to studies of cell cycle progression, cancer biology, and tumor suppressor signaling in murine cell lines and tissue lysates. Because RB1 phosphorylation status is widely used as a marker of proliferative state, this antibody is useful in experiments examining how growth factor stimulation, DNA damage, or oncogenic signaling alters RB1 mobility on SDS-PAGE gels. The polyclonal format offers recognition of multiple epitopes on the RB1 protein, which can be advantageous when probing samples where post-translational modifications might otherwise mask a single epitope. For best results, store refrigerated and avoid repeated freeze-thaw cycles to preserve antibody activity across the shelf life of the reagent.