Anti-Rb (P789) Antibody

This unconjugated rabbit polyclonal antibody from Antibodies.com is raised against the retinoblastoma (Rb) tumor suppressor protein at the P789 epitope region. The reagent is supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide as a preservative, with the solution buffered to approximately pH 7.2. Each 50 µl pack size is intended for research use only.

Validated for western blot applications, the antibody detects endogenous Rb in human, mouse, and rat sample lysates. The polyclonal format provides broader epitope coverage than monoclonal alternatives, supporting recognition of multiple regions across the full-length protein and aiding visualization of cell-cycle-regulated mobility shifts. As an unconjugated primary reagent, it pairs flexibly with HRP-, alkaline phosphatase-, or fluorophore-labeled secondary antibodies for chemiluminescent or fluorescent detection platforms.

Handling notes: the included sodium azide inhibits HRP activity if not removed before coupling or detection steps that are azide-sensitive; brief buffer exchange or thorough washing is recommended when required. The product is intended for laboratory research use only and is not validated for diagnostic or therapeutic purposes.