Anti-Rb Antibody

The Antibodies.com anti-Rb polyclonal reagent is raised in rabbit and affinity-purified against the retinoblastoma tumor suppressor protein (pRb/RB1), a 110 kDa nuclear phosphoprotein that governs the G1-to-S checkpoint of the cell cycle by sequestering E2F transcription factors. The unconjugated antibody is supplied as 100 µl of liquid antiserum applicable in western blot, immunohistochemistry, immunofluorescence, and ELISA workflows across research laboratories.

Validated reactivity across human, mouse, and rat tissue samples makes this polyclonal reagent a flexible option for comparative studies of cell-cycle regulation, tumor biology, and signaling pathways in which pRb status (phosphorylation at serine and threonine residues, loss of expression, or downstream E2F release) is being investigated. The unconjugated format permits direct conjugation in-house or pairing with a secondary antibody matched to the user's preferred detection chemistry.

For western blot, the antibody detects full-length pRb at approximately 110 kDa alongside faster-migrating hypophosphorylated species. In IHC and IF on fixed sections, nuclear staining is the expected pattern in proliferating cells, with loss of signal reported in many retinoblastoma and small-cell lung carcinoma samples. Each vial contains 100 µl of antiserum, with lot-specific QC data available from Antibodies.com.