Anti-Rb Antibody

Raised in rabbit against the retinoblastoma (Rb) tumor suppressor, this unconjugated polyclonal primary antibody is supplied by Antibodies.com as a 100 µl liquid aliquot intended for research use only. Validated across western blot, immunohistochemistry, immunofluorescence, and ELISA workflows, it detects endogenous Rb in lysates and sections prepared from human, mouse, and rat sources, giving cross-disciplinary value to cell cycle, cancer biology, and senescence research programs.

The Rb (pRb) target sits at the heart of the G1/S checkpoint, where phosphorylation controls E2F transcription factor release and entry into S phase. Investigators apply the reagent to compare pRb expression in tumor versus matched-normal tissue, to track differentiation in induced pluripotent stem cell and myoblast systems, and to monitor cell cycle re-entry after serum starvation or pharmacological arrest. Rb pathway inactivation is a defining feature of retinoblastoma and is also documented in small cell lung cancer, osteosarcoma, and certain triple-negative breast tumors, so the antibody is widely used in translational studies correlating RB1 status with therapeutic response.

For western blot, 20-40 µg of total protein per lane resolves full-length pRb near 110 kDa; paraffin-embedded sections pair well with citrate-based antigen retrieval for IHC. Short-term storage at 4°C suits working aliquots in a refrigerator, while longer term the vial should be kept frozen at -20°C, protected from light, and limited to a small number of freeze-thaw cycles to preserve binding activity.