Anti-RASGRF1 Antibody

This polyclonal IgG reagent from Antibodies.com targets RASGRF1 (Ras protein-specific guanine nucleotide releasing factor 1), a neuronal guanine nucleotide exchange factor that activates Ras family GTPases downstream of calcium/calmodulin and heterotrimeric G-protein inputs. Raised in rabbit and supplied as an unconjugated antiserum at 100 µl per vial, the antibody is validated for Western blot, immunofluorescence, and immunohistochemistry workflows on human and mouse specimens, supporting studies of synaptic plasticity, learning, memory consolidation, and tumor biology where RASGRF1 promoter methylation or expression status is a topic of active investigation.

Because the reagent is polyclonal, it recognizes multiple epitopes across the RASGRF1 protein, giving reliable detection in whole-cell lysates and fixed tissue sections; its IgG isotype is compatible with commonly used HRP, biotin, and fluorophore-conjugated secondary reagents, while the unconjugated format lets the laboratory select a detection chemistry matched to its assay platform. Investigators examining Ras-dependent MAPK signaling in neuronal, lymphoid, and epithelial model systems, including work on NMDA receptor coupling and Ras-driven transformation, can deploy this antibody for immunoblotting of cultured cell lines, immunofluorescent imaging of primary neurons, and IHC on archival tissue microarrays. Aliquot the antiserum on receipt, store refrigerated, protect from light, and limit freeze-thaw cycles to preserve activity across single-use experiments.