Anti-Ras Antibody

This recombinant rabbit monoclonal reagent from Antibodies.com targets the Ras small GTPase, a ~21 kDa membrane-anchored signaling protein encoded by HRAS, KRAS, and NRAS that couples receptor tyrosine kinase activity to the MAPK/ERK cascade. Mutations activating these isoforms drive constitutive downstream signaling and are found in roughly 30% of human cancers, including pancreatic, colorectal, and lung tumors, which is why Ras remains a heavily studied target in oncology, signal transduction, and drug discovery research.

The unconjugated antibody is supplied as 100 µl of liquid formulation in TBS buffer (pH 7.4) with 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative, shipped on cold packs. It has been validated across Western blot, immunoprecipitation, and flow cytometry workflows, and demonstrates reactivity with human, mouse, rat, and zebrafish samples, supporting cross-species studies in vertebrate model organisms.

Researchers commonly apply this reagent to probe Ras activation state via pull-down assays with Raf-RBD or RalGDS-RBD beads, to immunoprecipitate endogenous Ras-effector complexes from cell lysates, and to track Ras expression by flow cytometry in hematopoietic and tumor cell lines. Store refrigerated at 4 degrees C; aliquot to avoid repeated freeze-thaw cycles if extended use is planned.