Anti-RARS Antibody

The target RARS encodes arginyl-tRNA synthetase, a class I aminoacyl-tRNA synthetase that charges tRNA^Arg with L-arginine during cytosolic translation. This rabbit polyclonal reagent from Antibodies.com is supplied as 100 µl of unconjugated antiserum, validated for western blot and immunofluorescence workflows on human and mouse samples. It is commonly deployed in studies of protein synthesis fidelity, aminoacyl-tRNA synthetase biology, and hypomyelinating leukodystrophies linked to mutations in the gene.

Working concentrations typically require titration; diluents such as PBS or TBS with BSA and a non-ionic detergent are compatible with both blotting membranes and fixed cell preparations. For immunofluorescence, counterstaining with a DNA dye and an Alexa Fluor- or FITC-conjugated anti-rabbit secondary helps resolve the subcellular distribution of the arginyl-tRNA synthetase signal. Aliquot the vial upon first thaw if extended storage is planned, and limit freeze-thaw cycles. Sodium azide should be avoided in the buffer when planning downstream HRP-based western blot detection.