Anti-RARS Antibody

Arginyl-tRNA synthetase (RARS) catalyzes the ATP-dependent attachment of L-arginine to its cognate tRNA, an essential step that keeps cytoplasmic translation error-free. Raised in rabbit and supplied as an affinity-purified polyclonal reagent, this unconjugated Anti-RARS Antibody from Antibodies.com recognizes the endogenous human and mouse protein and is validated for western blot and immunohistochemistry workflows on cultured cells and fixed tissue sections. The vial contains 100 µl of antibody at 1 mg/ml in PBS supplemented with 0.1% sodium azide as a preservative and 50% glycerol as a cryoprotectant. For routine handling, keep the stock refrigerated between uses; for longer intervals the vial should be moved to -20 °C in working aliquots to limit repeated freeze-thaw cycles on the IgG fraction. Because the reagent is unconjugated, laboratories can pair it with their preferred anti-rabbit secondary, from HRP or alkaline phosphatase conjugates for chromogenic western blot and immunohistochemistry readouts to fluorophore-labeled secondaries for multiplex immunofluorescence. Researchers studying aminoacyl-tRNA charging, translation fidelity, and the broader family of cytoplasmic aminoacyl-tRNA synthetases use this reagent to track endogenous expression in human and mouse model systems, supporting western blot validation of knockdown and overexpression experiments and immunohistochemistry localizations in tissue panels relevant to translation biology and disease research.