Anti-RANKL (CD254) Antibody

Produced in rabbit hosts, this unconjugated polyclonal reagent from Antibodies.com targets RANKL (CD254), a TNF-family cytokine central to osteoclast differentiation, bone resorption, and lymph-node organogenesis. Researchers commonly deploy it in Western blot and immunohistochemistry workflows on human tissue and cell lysates to investigate osteoimmunology, metastatic bone disease, and TNF-receptor signaling through RANK, OPG, and TRAF6.

The vial contains 100 µl of affinity-purified antibody, ready to be diluted across multiple immunoblots or stained sections. For short-term use, refrigerate at approximately 4 °C; for longer storage, aliquot and freeze at –20 °C to minimize freeze-thaw loss of polyclonal titer. Reported reactivity covers human antigen, supporting studies of physiological and pathological bone remodeling, dental research, and tumor-bone interactions. The unconjugated format gives flexibility to pair the reagent with HRP, biotin, Alexa Fluor, or other secondary chemistries depending on the chosen detection method.