Anti-RANKL Antibody

This affinity-purified polyclonal reagent from Antibodies.com is raised in rabbit against RANKL (also known as CD254/TRANCE/OPGL), a key regulator of osteoclast differentiation and immune signaling. Generated from a synthetic peptide derived from the C-terminal region of human RANKL, the antiserum specifically detects endogenous levels of RANKL protein in samples of human, mouse and rat origin, and is supplied as a 50 µL aliquot at approximately 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The unconjugated antibody is validated for ELISA, Western blot, immunocytochemistry/immunofluorescence (ICC/IF) and immunohistochemistry, providing flexibility for both cell-based and tissue-based assays. For handling, short-term storage at 4 °C is acceptable, while long-term stability is maintained by aliquoting upon delivery and storing at –20 °C, avoiding repeated freeze/thaw cycles. Purified by affinity chromatography using an epitope-specific immunogen, this primary antibody is offered by Antibodies.com for research use only and serves as a versatile tool for laboratories investigating bone resorption, osteoimmunology and related signaling pathways.