Anti-RAN Antibody

Antibodies.com offers this rabbit polyclonal antibody raised against RAN, a ~25 kDa Ras-related GTPase that drives nucleocytoplasmic transport, mitotic spindle assembly, and nuclear envelope reformation. The reagent is supplied as a 200 µl unconjugated IgG preparation validated for western blot, immunohistochemistry, and flow cytometry on human and mouse samples. RAN sits at the core of importin-mediated cargo shuttling between cytoplasm and nucleus, partnering with RCC1, RanGAP, and RanBP effectors to establish the RanGTP-RanGDP gradient that directs karyopherin trafficking. Investigators studying cell cycle progression, nuclear import kinetics, mitotic checkpoint control, or altered transport in oncogenesis use this antibody to probe expression and subcellular localization across cell lines and tissue sections. Polyclonal reactivity broadens epitope coverage, which is helpful for detecting truncated RAN constructs, splice isoforms, or partially degraded material in cell lysates. For best results, aliquot upon arrival, store refrigerated, and protect from repeated freeze-thaw cycles to preserve binding activity across experiments.