Anti-RAF1 (phospho S259) Antibody

Targeted at the Ser259-phosphorylated form of the RAF1 (C-RAF) serine/threonine kinase, this rabbit polyclonal IgG reagent from Antibodies.com is supplied as 50 µl of unconjugated antibody in a single vial. The antibody was raised against a synthetic phosphopeptide derived from the region of human RAF1 surrounding the Ser259 phosphorylation site, and it recognizes the kinase only when that residue is phosphorylated, yielding the expected band near 73 kDa on Western blot.

The reagent is affinity-purified by epitope-specific chromatography and reaches >95% purity by SDS-PAGE, and it is formulated at 1 mg/ml in phosphate-buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2. Validated for Western blot on human samples, it is routinely paired with a pan-RAF1 antibody so that phospho-S259 occupancy can be read against total kinase levels in whole-cell lysates from cultured cell lines, tumor-derived material, or stimulated primary cells. Aliquot on first use, refrigerate on receipt, and protect from repeated freeze-thaw cycles to preserve phospho-epitope reactivity across multiple experiments.