Anti-Raf1 (Ab-338) Antibody

This polyclonal antibody from Antibodies.com targets a specific phosphorylation site (residue 338) on Raf1, a MAP kinase kinase kinase that sits upstream in the Raf/MEK/ERK signaling cascade. The reagent is raised in rabbit and is supplied as an unconjugated IgG preparation in a 100 µl vial, giving laboratories a flexible detection reagent for both western blot and immunohistochemistry workflows on human, mouse, and rat tissue or cell samples.

Because the epitope centers on the Ab-338 phospho-acceptor, the antibody is most often used to probe activated Raf1 rather than total protein levels. Researchers studying growth-factor-driven ERK pathway activity, oncogenic Ras signaling, or downstream MAPK biology will pair the reagent with western blot lysate analysis, while immunohistochemistry applications on formalin-fixed sections allow spatial mapping of active Raf1 in tissue contexts. Aliquot on receipt and store at -20 °C to preserve phospho-specific binding; avoid repeated freeze-thaw cycles, and protect from light when handling working dilutions.

The unconjugated format lets end users choose their own secondary reporter, whether HRP, biotin, or a fluorophore, and the polyclonal backbone offers redundancy across the immunogen for stronger signal on low-abundance targets. Researchers should still validate the reagent in their own hands because phosphorylation-state recognition is sensitive to fixation, detergent lysis, and phosphatase inhibitors present during sample preparation.