Anti-Raf1 (Ab-259) Antibody

Raised in rabbit against a synthetic peptide surrounding residue 259 of the Raf1 kinase, this unconjugated polyclonal antibody targets a region of the MAPK/ERK pathway effector that includes a key regulatory phosphorylation site. It is part of the Antibodies.com primary antibody line intended for basic-research use, supplied as 100 µl of liquid stock in a single vial and ready for dilution into standard immunodetection workflows.

Validated applications are Western blot and immunohistochemistry, with demonstrated reactivity against human, mouse, and rat samples. The polyclonal format supports detection of full-length Raf1 protein in cell lysates and in fixed tissue sections, while the unconjugated conjugate lets the end user pair it with a secondary antibody of choice (HRP, fluorophore, or biotin) for the preferred detection read-out.

Common research contexts include growth-factor signalling studies, Ras-Raf-MEK-ERK cascade activity monitoring, and phosphorylation-state analysis at the Ser259 regulatory site, which is constitutive in resting cells and lost upon pathway activation. The reagent is intended for research use only and is not approved for diagnostic or therapeutic applications.