Anti-Raf-B (phospho-S445) Antibody

Raised in rabbit, this unconjugated polyclonal IgG recognizes the phosphorylated form of human, mouse, and rat B-Raf (also called Raf-B) only when serine 445 carries a phosphate group, allowing direct visualization of kinase regulation within the canonical RAF-MEK-ERK cascade by Western blot. Stocked at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a 100 µl vial, the reagent from Antibodies.com tolerates standard freeze-thaw handling when aliquoted and stored frozen, supporting reproducible stripping and reprobing workflows on the same membrane.

Phosphorylation at S445 lies within the B-Raf kinase domain and reflects upstream signaling through the Ras-RAF-MEK-ERK cascade, so this reagent is particularly useful for tracking pathway reactivation following growth factor stimulation, serum starvation, or inhibitor washout in cell-line and primary-cell models. The polyclonal format improves confidence in detecting the modified site across B-Raf isoforms, while the unconjugated presentation allows pairing with HRP- or fluorophore-labeled secondary antibodies to match existing imaging platforms without signal interference from a pre-conjugated reporter.