Anti-Raf-B Antibody

This polyclonal antibody from Antibodies.com is raised in rabbit and targets the Raf-B protein (also known as BRAF), a serine/threonine kinase that operates within the MAPK/ERK signaling cascade. Validated for western blot and immunohistochemistry workflows, the reagent detects human, mouse, and rat Raf-B, supporting comparative pathway studies in cell line and tissue-based models. The unconjugated reagent is supplied as 100 µl of a 1 mg/ml stock formulated in PBS with 0.1% sodium azide and 50% glycerol. Aliquot upon arrival and store at -20°C in single-use fractions to preserve activity across multiple freeze-thaw cycles; protect from light. Because B-Raf sits upstream of MEK1/2 and ERK1/2, this antibody is useful in research into oncogenic MAPK signaling, BRAF V600E-driven tumor biology, melanocyte and neuronal development, and differentiation studies mapping Raf-B expression across normal versus transformed tissue. The polyclonal format recognizes multiple epitopes on the target, which can give a stronger overall signal in WB and IHC than a monoclonal reagent.