Anti-Raf-1 (R282) Antibody

Reagent for MAPK Cascade Research

This unconjugated rabbit polyclonal antibody from Antibodies.com targets the R282 epitope in the regulatory domain of Raf-1, a serine/threonine kinase central to the Ras-Raf-MEK-ERK signaling cascade that governs cell proliferation, differentiation, and survival. Supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 15 mM sodium azide at approximately pH 7.2, the 100 µl vial is ready for use in western blot (WB) and immunohistochemistry (IHC) workflows.

Validated for reactivity against human, mouse, and rat samples, this reagent supports investigation of Raf-1 expression, post-translational modification, and pathway activation across cell line, primary cell, and tissue contexts. Researchers in oncology, developmental biology, and neuroscience use Raf-1 antibodies to probe mitogenic and stress-activated MAPK signaling, including studies of Ras-driven transformation, ERK feedback loops, and kinase inhibitor response.

The polyclonal format provides recognition of multiple epitopes on Raf-1, which can improve signal robustness in immunoblotting of total and phosphorylated species. Standard pairing includes RIPA or NP-40 lysate preparation, SDS-PAGE separation, PVDF or nitrocellulose transfer, and enzymatic or fluorescence-based detection, while IHC applications benefit from antigen retrieval on formalin-fixed paraffin-embedded sections.