Anti-Raf-1 (phospho-S621) Antibody

Phosphorylation at serine 621 modulates the activation state of Raf-1, a MAP3K positioned at the entry point of the Ras-Raf-MEK-ERK cascade that governs mitogenic and survival signaling. The Antibodies.com polyclonal reagent specifically recognizes the phospho-S621 form of Raf-1, allowing researchers to distinguish phosphorylated pools from total protein in cell-based assays.

Raised in rabbit and supplied as 100 µl at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, this unconjugated antibody is validated for Western blot and Immunofluorescence against human samples. The polyclonal format broadens epitope coverage around the modified residue, supporting detection across denaturing SDS-PAGE separations and fixed-cell imaging workflows.

Investigators studying RAF1-driven oncogenic signaling, receptor tyrosine kinase output, or pathway crosstalk can monitor S621 phosphorylation dynamics in response to growth factors, pharmacological inhibitors, or activating mutations. The glycerol-containing buffer permits frozen storage with minimal freeze-thaw cycling; aliquoting on first use preserves phospho-specific activity. This reagent is intended for research use only.