Anti-Raf-1 Antibody

Raf-1 is a cytoplasmic serine/threonine kinase that operates within the MAPK/ERK cascade, transducing signals from activated Ras to the MEK/ERK module and thereby controlling cell proliferation, differentiation, and survival. Antibodies.com provides this rabbit polyclonal anti-Raf-1 antibody as a 100 µl aliquot at 1 mg/ml in PBS supplemented with 0.1% sodium azide and 50% glycerol, an established cryoprotective storage buffer that preserves antibody activity across freeze-thaw cycles. The unconjugated reagent is validated for Western blot (WB) and immunofluorescence (IF) workflows and reacts with samples from human, mouse, and rat, making it broadly useful for comparative studies across model systems and primary tissue. Because the antibody is polyclonal, it binds multiple epitopes on the ~73-74 kDa Raf-1 protein, supporting reproducible detection even when expression levels are low or when the target carries post-translational modifications. Researchers in cancer biology, signal transduction, and developmental disease (including Noonan syndrome, where germline Raf-1 mutations are causative) routinely rely on this reagent to characterize pathway dysregulation, map subcellular localization of Raf-1, and confirm gene-silencing or pharmacological inhibition experiments. The formulation supports straightforward downstream handling: working stocks can be diluted into assay buffer, and the remaining concentrate should be stored refrigerated at 4 degrees C for short-term use or frozen for longer-term retention without loss of titre.