Anti-RAD9A Antibody

RAD9A is a key component of the 9-1-1 checkpoint complex, a heterotrimeric clamp that loads onto DNA at sites of damage to initiate the ATR-dependent signaling cascade that halts cell cycle progression and supports repair.

Antibodies.com supplies this Rabbit polyclonal reagent raised against the human RAD9A protein, delivered as 100 µL of unconjugated antisera validated for Western blot and immunofluorescence on human samples. The polyclonal format offers recognition of multiple epitopes on the target, which is useful when monitoring post-translationally modified forms or alternative splice variants of RAD9A in lysates and fixed cells.

Because RAD9A acts upstream of CHK1 phosphorylation and downstream of ATR recruitment at single-stranded DNA, researchers studying replication stress, genotoxic responses, and ATM/ATR pathway activation use this antibody to track checkpoint engagement in cultured cells and tissue sections. For best results, aliquot upon arrival, store refrigerated to preserve activity across repeated freeze-thaw cycles, and dilute into an appropriate blocking buffer for immunofluorescence or standard blotting protocols. Intended for research use only.