Anti-RAD9A Antibody

RAD9A is a key component of the 9-1-1 checkpoint complex that senses DNA damage and activates ATR-mediated signaling cascades controlling cell cycle arrest and repair decisions. The unconjugated rabbit polyclonal antibody offered by Antibodies.com enables detection of endogenous human RAD9A across multiple experimental platforms, including Western blot analysis of cell lysates, immunofluorescence staining of fixed cells, and immunohistochemistry on tissue sections.

Supplied in a vial as 100 µl of a 1 mg/ml stock in PBS with 0.1% sodium azide and 50% glycerol, this polyclonal reagent tolerates standard freeze-thaw cycles and is best stored frozen at -20°C in working aliquots to preserve activity. Researchers investigating genotoxic stress responses, replication fork stability, and S-phase checkpoint integrity use this reagent to monitor RAD9A recruitment to damage sites and to probe alterations in 9-1-1 complex assembly following treatment with DNA-damaging agents such as UV, hydroxyurea, or cisplatin.

Intended for research use only, the antibody is validated for human samples and is commonly applied in studies of cancer cell resistance to chemotherapy, Fanconi anemia pathway crosstalk, and programmed cell death signaling downstream of persistent DNA lesions.